We offer a fully-funded PhD position in the Castro Lab in the Biosciences Department, CHMLS. The Castro lab focuses on a cell structure, the nuclear pore complex (NPC), and explores how components of the NPC affect pore structure and function. The NPC’s primary function is the import/export of molecules between the cytoplasm and the nucleus (and vice-versa). Protruding into the nucleus, the NPC nuclear basket, functions beyond import/export, interacting with the genome.
The research project, funded by the College of Health, Medicine and Life Sciences and the Academy of Medical Sciences, aims to investigate a particular component of the NPC, Nup153, and how modifications of this protein can affect NPC structure and import function. We are particularly interested in how Nup153 affects the import of big molecules like viruses, particularly HIV-1. This project involves collaborations with experts in the field, namely Dr Shytaj (Bristol University) and Prof Padilla-Parra (KCL).
We invite interested candidates to apply by 4 pm (UK time), Friday, 18 July 2025. Due to our funding restrictions, this opportunity is open only to UK candidates or those with settlement status. Interviews will be conducted in July. Start date is 1st October 2025.
Based in the College of Health, Medicine and Life Sciences, this studentship offers a full-time annual London rate stipend estimated at £22,780 (including London weighting) and Home fees for a maximum of 36 months.
The Nuclear Pore Complex (NPC) is one of the most intricate and essential structures in the cell, forming a gateway through the nuclear envelope that regulates the exchange of molecules between the nucleus and cytoplasm. Beyond this canonical role, the NPC is exploited by certain viruses, including HIV-1, which must traverse the nuclear envelope to integrate into the host genome—a critical step in its life cycle.
This project will investigate post-translational modifications of Nup153, a key component of the NPC nuclear basket, and how they affect both the structural organisation of the NPC and its import functions, particularly in the context of HIV-1 nuclear entry. Using biochemical, molecular, and cellular approaches, the student will dissect the impact of Nup153 modifications on NPC function and assess how these changes influence the efficiency of HIV-1 nuclear import.
The research will involve collaborations with leading virologists at the University of Bristol (Dr Shytaj) and King’s College London (Prof Padilla-Parra), providing a multidisciplinary training environment at the interface of nuclear biology and virology.
For informal discussions, please contact Dr Ines Castro on ines.castro@brunel.ac.uk.
Eligibility
Candidates should have an undergraduate degree (first or upper second class) or equivalent qualification in biosciences or a related field. A Masters qualification in a relevant area and laboratory experience is desirable. Previous Experience in molecular biology (cloning/qPCR), biochemistry (Western Blots, Immunoprecipitations) and mammalian cell culture are desirable criteria. Applicants who have not been awarded a degree by a University in the UK will be expected to demonstrate English language skills to IELTS 7.0 (minimum 6.5 in any section).
How to apply
If you wish to apply, please e-mail the following to chmls-pgr-officestaff@brunel.ac.uk by 4pm (UK time) on Friday 18 July 2024.
· An up-to-date CV.
· A single-page A4 single-spaced personal statement describing why you are a suitable candidate (i.e. outlining your qualifications and skills).
· One example of your academic writing (e.g. an essay, a section from a dissertation).
· A summary of your teaching experience or your willingness to support teaching activities.
· Names and contact details for two academic referees.
· A copy of your highest degree certificate and transcript.
· A copy of your English language qualification, where applicable.
Short-listed applicants will be required to attend an interview week commencing 30 June 2025.
For further information about how to apply, please contact the College of Health, Medicine and Life Sciences Postgraduate Programmes Office on chmls-pgr-officestaff@brunel.ac.uk.
